Chondrogenic potential of chick embryonic calvaria: I. Low calcium permits cartilage differentiation
نویسندگان
چکیده
منابع مشابه
Monomeric and aggregate proteoglycans in the chondrogenic differentiation of embryonic chick limb buds.
Proteoglycan heterogeneity was studied during the in vivo differentiation of embryonic chick limb cartilage. Recently, it has been shown that during the differentation of limb cartilage the proportion of the aggregated form of proteoglycans increases whereas the unassociated monomeric forms decrease, and this has been related to the synthesis of two link proteins at a specific stage of differen...
متن کاملHypoxic chondrogenic differentiation of human embryonic stem cells enhances cartilage protein synthesis and biomechanical functionality.
BACKGROUND Engineering musculoskeletal cartilages with stem cells remains a challenge because researchers must control many factors, including differentiation and cartilage matrix synthesis, particularly collagen II production. Hypoxia has effects on many cellular processes, though few investigations with hypoxia provide quantitative functional data on engineered cartilage. OBJECTIVE This stu...
متن کاملEnzyme Activities in Chick Embryonic Cartilage
Some characteristic enzymatic activities were determined in chick embryonic cartilage and compared with the analogous activities in bone and liver. Chondrocytes were isolated, broken by sonication, and subjected to subcellular fractionation to yield a nuclear pellet, the mitochondrial, lysosomal, and microsomal fractions, and the high speed supernatant solution. It was established that these fr...
متن کاملBioluminescence Assays for Monitoring Chondrogenic Differentiation and Cartilage Regeneration
Since articular cartilage has a limited regeneration potential, for developing biological therapies for cartilage regeneration it is important to study the mechanisms underlying chondrogenesis of stem cells. Bioluminescence assays can visualize a wide range of biological phenomena such as gene expression, signaling, metabolism, development, cellular movements, and molecular interactions by usin...
متن کاملThe sulfation of chondroitin sulfate in embryonic chick cartilage epiphyses.
With the use of purified chondroitinase of Proteus vulgaris, a method has been devised for the determination of relative amounts of chondroitin, chondroitin-4-SO*, and chondroitin6-SO4 in cartilage. This method was applied to the analysis of embryonic chick epiphyseal cartilage. With 35S04 and ‘*C-acetate it was possible to study the relationship of embryo age to rate of formation of chondroiti...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Developmental Dynamics
سال: 1995
ISSN: 1058-8388
DOI: 10.1002/aja.1002020103